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Separation of Insulin and Partial Reduction Products



Column: Vydac 218TP, C-18, 5µm, 4.6 x 250mm
Order No.: 941124
Eluent A: 0.1% TFA
Eluent B: 0.092% TFA in 60:40 ACN:H2O
Gradient: from 35-85% in 25 min at 1.0mL/min
In a study of bridged peptides, disulfide bonds were reduced and the reduction products were separated from normal insulin by C18 reverse-phase HPLC.

HPLC Chromatograms

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