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Separation of Protein Standards

  1. Ribonuclease A (2µg)
  2. Cytochrome C (2µg)
  3. Lysozyme (2µg)
  4. BSA (2µg)
  5. Myoglobin (2µg)
  6. Ovalbumin (2µg)


Column: Cosmosil 5C18-AR-300, 4.6 x 150mm
Order No.: 930777
Eluent A: 0.05% TFA/20% Acetonitrile
Eluent B: 0.05% TFA/60% Acetonitrile, A 100% to B 100%
Gradient: Linear, 20 min
Temperature: 30°C
Flow Rate: 1.0mL/min
Detector: UV 220nm

HPLC Chromatograms

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