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Peptides and Proteins

Column: XPERTEK Kromasil, C-8, 5µm, 4.6 x 250mm
Mobile Phase A: 0.1% TFA in Acetonitrile:Water (10:90)
Mobile Phase B: 0.1% TFA in Acetonitrile:Water (90:10)
Gradient Time: 0 0%B, 8 25%B, 20 75%B
Flow Rate: 2mL/min
Detector: UV 254nm

Protein/Peptide Mixture

Column: Vydac 219TP, Diphenyl, 5µm, 4.6 x 250mm
Order No.: 941130
Eluent: Gradient 0-70% ACN with 0.1% TFA in 67.5 min

HPLC Chromatograms

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