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Nucleotides

  1. 5'-AMP
  2. 5'-GMP
  3. 5'-IMP
  4. 3'-AMP
  5. 2'-AMP
  6. 5'-TMP
  7. 3',5'-cAMP
  8. 5'-ADP
  9. 3',5'-cGAP
  10. 5'-GDP
  11. 5'-ID
  12. 5'-TDP


Column: XPERTEK Inertsil, ODS-2, 5µm, 4.6 x 150mm
Eluent A: 5mM Tetrabutylammonium Br + 20mM K2HPO4 buffer (pH 3.5)
Eluent B: 5mM Tetrabutylammonium Br + 20mM K2HPO4 buffer 60% CH3CN (pH 3.5); A/B 95/5 -> (25 min) -> 50/50
Temperature: RT
Flow Rate: 1.0mL/min
Detector: UV 260nm








Nucleotides

  1. CMP
  2. UMP
  3. GMP
  4. AMP
  5. ADP
  6. ATP


Column: Ultrasphere ODS, C-18, 4.6 x 250mm
Order No.: 931002
Mobile Phase A: 50mM triethylammonium phosphate, pH 7.0
Mobile Phase B: 50mM triethylammonium phosphate, pH 7.0 in 20% Acetonitrile
Flow Rate: 1.0mL/min
Detector: 254nm








Optimized Separation of 5' Nucleotides

  1. CMP
  2. AMP
  3. UMP
  4. GMP
  5. CDP
  6. ADP
  7. UDP
  8. CTP
  9. GDP
  10. ATP
  11. UTP
  12. GTP


Column: PL-SAX 1000Å, 8µm, 4.6 x 50mm
Order No.: 946581
Eluent A: 0.02M KH2PO4, pH 2.6
Eluent Phase B: 0.5M KH2PO4, pH 3.5
Gradient: Linear O - 100% B in 20 min.
Flow Rate: 1.0mL/min
Detector: UV, 260nm

HPLC Chromatograms

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