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Water Soluble Vitamins

  1. Pyridoxine HCL
  2. Thiamine HCL
  3. Niacinamide
  4. Folic acid
  5. VIT B12
  6. Riboflavin


Column: XPER-CHROM HL, C18, 5µm, 4.6 x 250mm
Order No.: 932223
Mobile Phase: A: 0.5m potassium acetate, pH 5, B: Acetonitrile
Gradient: 2.5 to 30% B


Water Soluble Vitamins

  1. L-ascorbic acid
  2. Nicotinic acid
  3. Nicotinamide
  4. Pyridoxal
  5. Pyridoxamine
  6. Pyridoxine
  7. Thiamine
  8. Folic acid
  9. Cyanocobalamin
  10. Riboflavin

Column: XPERTEK Inertsil, ODS-2, 5µm, 4.6 x 150mm
Eluent A: 5mM IPCC-05 + 0.1% H3PO4
Eluent B: 5mM IPCC-05 + 0.1% H3PO4 in 80% CH3CN, A/B 97.5/2.5 -> (20 min) -> 50/50, IPCC-05: Pentanesulfonate Na
Temperature: 40°C
Flow Rate: 1.0mL/min
Detector: UV 254nm

Water Soluble Vitamins

  1. Vitamin C
  2. Niacin
  3. Pyridoxine (B6)
  4. Thiamine (B1)
  5. Nicotinamide (B3)
  6. Folic acid (M)
  7. Cyanocobalamin (B12)
  8. Riboflavin (B2)

Column: Vydac 201HS, C-18, 5µm, 4.6 x 250mm
Order No.: 941112
Eluent: Gradient from 2.5 to 50% ACN with 0.1M KOAc, pH 5.2 over 15 min at 1.5mL/min

HPLC Chromatograms

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