Quality Chromatography & Life Science Products
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Quality Chromatography & Life Science Products |
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Multiple Affinity Removal Spin Cartridges AGILENT 07-08 Catalog
Click Cover P.J. Cobert Associates, Inc. 2302 Weldon Parkway St. Louis, MO 63146 Toll Free: 1-800-972-4766 Phone: 314-993-2390 Fax: 314-993-2491 Over 30 Years of chromatography experience! | FILTERS Technical Information ______________________________________________________________________________________________ MEMBRANE SELECTION Regenerated Cellulose (RC) Regenerated Cellulose is a hydrophilic, solvent-resistant low protein binding membrane. RC membrane is ideal for removing particulates from HPLC samples, prior to injection. This membrane is compatible with all HPLC solvents, and can be utilized for particle removal and de-gassing of these solvents. RC membranes are also compatible with aqueous solutions in the pH range of 3 to 12. Extractables with water are less tha 1%. Regenerated Cellulose membranes exhibit low non-specific adsorption thus they are well suited for filtration of biological samples where maximum recovery of protein is important. When used with a glass pre-filter in the same housing, this membrane is ideal for filtration of tissue culture media, as well as general biological sample filtration. Filters containing RC membrane are available as non-sterile or sterile and pyrogen free. RC membranes can be sterilized by gamma radiation, autoclaving, ethylene oxide or by dry heat.
Cellulose Acetate (CA) Cellulose Acetate is a very low protein binding membrane that is ideal for aqueous based samples. CA membranes are an excellent choice when maximum protein recovery in the filtrate is critical. Laboratory studies show that CA membranes bind less protein than PVDF or Polysulfone membranes. When used with a glass pre-filter in the same housing, these membranes are ideal for filtration of tissue culture media and sensitive biological samples. The pre-filter increases yield.
Nylon New Low Extractable Nylon membranes combine the solvent resistance of Nylon with a membrane that exhibits very low extractables. Nylon is commonly used for general laboratory filtration and filtration of HPLC samples prior to injection. Nylon binds protein and should NOT be used when maximum protein recovery is important. Nylon can be sterilized by autoclaving at 120°C, gamma radiation or ethylene oxide.
Glass Media Glass Media membranes are commonly used as pre-filters in many filtration devices. Specialized glass membranes are used for DNA recovery and clean-up.
Polyvinylidene Difluoride (PVDF) PVDF (polyvinylidene difluoride) is a hydrophilic, solvent-resistant membrane that exhibits low levels of UV-absorbing extractables. PVDF is useful for HPLC sample filtration as well as general biological filtration. PVDF is considered to be one of the low protein binding membranes.
Polyethersulfone (PES) Polyethersulfone is a hydrophilic membrane with high flow characteristics. PES membranes are moderate protein binding membranes. Solvent resistance is fair.
PTFE Polytetrafluoroethylene is hydrophobic and chemically resistant to all solvents, acids and bases. PTFE membrane does not impart any extractables to the filtrate. PTFE is an ideal membrane for transducer protectors since it blocks water vapor. PTFE is ideal for filtering and degassing chromatography solvents.
Cellulose Nitrate (CN) Cellulose Nitrate membranes are hydrophilic with limited solvent resistance. CN membranes are high protein binding and are ideal for DNA & RNA blotting.
Ultrafiltration Ultrafilter membranes are also known as Molecular Weight Cut Off (MWCO) membranes. Ultrafilter membranes are special membranes that separate various compounds by the molecular weight of the compound. UF membrane sizes range from 5000 to 100000 Dalton. Since these membranes have extremely small pore sizes (0.0001µ for a 5K membrane and 0.01µ for a 100K membrane) they are very useful for the concentration of various proteins as well as removal of salts. UF membranes are used for the recovery and concentration of yeast virus and enzymes from biological samples. These membranes are very useful for removing primers and salts from PCR reaction mixtures. UF membranes can be used for clean-up and recovery of low molecular weight materials from culture media fermentation broths, or cell lysates. These membranes are available in centrifugal devices and in 25mm syringe filter housings. The centrifugal devices should always be used with fixed angle rotors and never with swinging bucket rotors. UF membranes will prematurely clog and cease to function in swinging bucket rotors. It should be noted that sterile untrafilters have a limited shelf life while non-sterile filters have a long shelf life. ______________________________________________________________________________________________ FILTER HOUSING SELECTION
Modified Acrylic (MA) Modified Acrylic housings are to be used only with aqueous based samples. Even low concentrations of solvents will destroy Acrylic housings. The modified Acrylic resin used is USP XXII Class VI plastics approved and has passed all the tests for cytotoxicity. This ensures that no cytotoxins that could cause cell death will leach from these filter housings. This resin is also gamma radiation resistant and does not discolor when sterilized.
Polypropylene (PP) Polypropylene housings are solvent resistant and exhibit low extractables. PP housings combined with low extractable Nylon, Regenerated Cellulose or PTFE membrane, are ideal for filtering solvents and samples for chromatography. All housings are molded with FDA approved food contact grade PP resin.
Tefzel® (E-TFE) Tefzel syringe filter housings with non-supported PTFE membranes are excellent for critical analytical work that demands zero extractables.
Note: All Colorants used in housings are FDA approved food contact grade. All filters are for laboratory and research use only.
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